Wednesday, October 13, 2010
brooke
Tuesday, October 12, 2010
i'm poor
schto eto za research?
Thursdays: i set up fish, by separating out mating pairs into separate breeding tanks, and letting them love each other overnight.
Fridays: I take the fish down, by pouring the fish back into their main tank and then pouring any embryos into small petrie dishes for cleaning and sorting. the embryos are kept separate from the fish in the tanks because they fall through a perforated inner tank and rest on the bottom where the fish can't get at them, because they will eat them. after the eggs are all harvested (there are usually hundreds), about 100-120 are separated out and rinsed, getting rid of any unfertilized or rotting embryos, and any that might be showing signs of abnormal development.
Mondays: after the embryos have been sitting in an incubator over the weekend, they are now little swimmers with eyes, a pumping heart, immune system, and circulatory system. it's crazy how quickly they develop. if you've got 15 minutes, you can actually watch cell division. it will change your life. at this point, i soak the fish in a certain drug that we are using and let them swim around a little in it for like an hour, and then i make a small wound in the back part of the feathery part of their fin, which begins an inflammatory response. i let them sit in the drug with these wounds for a couple of hours and then fix them in a formaldehyde solution that crosslinks all of the cellular components to freeze them all at that moment in time.
Tuesdays: i wash the embryos 3 times for 5 minutes and soak them in a block solution to prepare them for their first antibody. this antibody binds to certain cellular components and will give a place for a second antibody to bind the next day.
Wednesdays: the embryos are washed 4 times for 20 minutes each time, and then soaked in a second antibody that has a pink fluorescent tag on it that will allow all of the leukocytes to show up under fluorescent microscopy.
Thursdays: fish are setup for the next week, and the fish from the current week are washed 4x20 and then run through a glycerol series of 30% glycerol, 50%, and 80% and left in the 80% overnight.
Fridays: fish are taken down, and the current fish get their heads separated from their tails, and the tails are arranged on a microscope slide, and then examined under a fluorescent microscope. under one filter, you can see all of the leukocytes (white blood cells) and i count them. under a different filter i see only neutrophils, which are a specific white blood cell that has been genetically altered to carry a gene for a fluorescent green protein called GFP. these glow green under the different filter, so i can differentiate how many of the total leukocytes are neutrophils. i can then compare counts with the control slide, to the counts from the slides from fish at different drug concentrations, and see if the drugs have any sort of effects in the inflammatory response.
as i've been doing this process i started getting frustrated with the lack of accuracy and repeatability with the experiments and decided i would start messing around with it and testing different things. so i started making things really sterile with some of the fish, bleaching them and putting them in filtered water. some of the other fish, i soaked in bacteria rich water, made from rotting fish embryos. the surprising results are that the bacteria water, drastically increased neutrophil counts, while reducing macrophage counts. this was something no one expected, and i'm doing other tests to fine tune things. but to make a long story short (or not as long), the lab is potentially going to redo how they perform their experiments to reflect these findings. everyone is all excited about it, so it's pretty exciting, but now it puts a lot of extra pressure on me to get this figured out. crazy, but i'm loving research.
Bring on the fall
end of sums
Thursday, August 12, 2010
trax gallery
here we have the motley crue fan club manager with his friday the 13th tattoo. he was on his way to adopt a puppy.
this average looking guy has more fun going on with the back of his hair than i have all week. plus grey sideburns.
in case you can't see it, her tattoo says "rick" which i'm always a big fan of.
this guy has some major inner thigh fitness going on.
Friday, July 30, 2010
end of summer semester
Tuesday, July 20, 2010
7/2
Wednesday, July 7, 2010
7/7
Tuesday, July 6, 2010
4th of july weekend
2nd physiology exam
Wednesday, June 30, 2010
pigeon poop
6/30
Tuesday, June 22, 2010
sleep disorder
Sunday, June 20, 2010
karate kid
gi joe
Saturday, June 19, 2010
Friday, June 18, 2010
1st round summer tests
Wednesday, June 16, 2010
run forrest
Sunday, May 23, 2010
zebrafish surgery
and...i'm back in school again
summer break
Wednesday, May 5, 2010
finals week
Sunday, May 2, 2010
tiny dancer
NOOOOOOOOOOOOOOOOOOOOOOOO!
leaderSHAPE
santa hits the ER
p.s. actually now that i look at this photo full size, i guess he wasn't distracted. he was looking right at me. it's a little scary...those eyes...that beard...those little christmas ornaments...if santa tracks me down and kills me, someone give this picture to the police.
Calculicious
Bio Exam
Mr. Vice President
Sunday, April 11, 2010
beard
a lone lonely loner
physician shadowing
Saturday, March 27, 2010
my parents
my poor colon
brianhead
Sunday, March 21, 2010
spring break '10
time rolls by...so slowly
chem exam
the 811 acrobat
Sunday, March 14, 2010
loves of my life
Lava Hot Springs
a week of exams
TRAX accident (TRAXident)
AED and old ladies
also, we had dr. samuelson's lecture on personal statements this week. i couldn't make it because of my bio lab, but we had our new signs out advertising it, and that was pretty exciting to see, i must say. they turned out really well. everyone stole the flyers out of them at first, but then we made new flyers with "do not remove" on them, and that did the trick. add a member meeting in there on monday, and it was a full week of AED. but it was actually kind of nice to get to know everyone better. one girl whipped out her guitar and started singing, the student advisor was trying to hook up with the old ladies...lots of fun.
Saturday, March 6, 2010
so many exams, so little time
Saturday, February 27, 2010
the poster is done!
don't people have jobs?
i find myself getting very annoyed at different people. my patience for idiots is really in short supply these days. the group i will focus on now are the computer lab rats. i get so annoyed when i need to get on a computer and there are none available because everyone is on facebook or chatting with girls in singapore. anyway, there are 3 guys in the downstairs computer lab who are like 50 plus, and they seriously get there by like 7:30 in the morning and are there until like 6:30 at night and all they do is check email and watch tv shows and movies on the computer. ALL DAY LONG!!! the worst one is this guy, who actually brings a bag full of food and drinks and just camps out all the live long day. there is this cloud of smell like old cheese around him, and i just find myself getting mad every day that i see him.
Tuesday, February 23, 2010
brain fry
Friday, February 19, 2010
public transportation
I need sleep
okay, so just a disclaimer: for those not in the know, i don't capitalize or use a lot of punctuation, and i don't intend to start now.
so i've decided to start a blog to catalog the adventures (or misadventures) of a non-traditional, mid-thirties premed student, with a wife and four kids. this is something that doesn't happen to most people, and something i actually feel very privileged to be a part of. at 34, soon to be 35, who gets the opportunity to learn biology, and chemistry, and calculus. i used to worry about when my kids would bring home math homework, about not being able to help them. i don't worry about that anymore. instead i worry about being home to help them with it. and that is the hardest part about what i'm doing. i miss my family. i used to be in my underwear, all day, with my kids coming down and bugging me in my office, and hearing them jump from the kitchen counter and having the lights shake in my office as they would crash on to the floor. now, i'm settling for conversations via skype in the morning and at night, on particularly long days.
just for the record, this is my general schedule:
wake up: 5:20am
catch bus: 6:07am
study on bus: 6:07am-7:15am
study at library: 7:30am-9:30am
classes and study: 9:30am-6:45pm (or 9:30am-8:45pm tuesday and wednesday)
catch train and study all the way home: 6:45pm-8:30pm (or 8:45pm-10:30pm T and W)
when i get home, its like a little vacation every night. if the kids are up, i play with them and hear countless stories about all the important things in their days. we read scriptures and say family prayers, and they are off to bed. if they are already asleep, the best i get is a half-conscious "hey dad" and a not great smelling kiss. but i'll take it. then my wife and i get some time together. finally. the late night meals are not ideal, but its all we can do. that is my prized time, just laying on the couch, watching our favorite shows, pausing every five minutes to analyze "survivor" strategy, or for me to tell her some uninteresting story about school, that i can tell is making her eyes glaze over. she's got plenty of those same stories too. sometimes we pretend we're interested, sometimes we don't. we're honest like that. but that's what all this time away gives me: an appreciation of what i have.
so here's what i have: an eight year old minivan, and a '64 chevy impala. we love our cars, and don't need anything more. our '08 gmc yukon xl, is a distant memory (even the black rims). why did we need all that? who knows. we spilled a can of paint in the back of the minivan and didn't even blink an eye. its just a funny, thick, black, messy memory. we live in a basement of my parents second home in highland. somehow we crammed all of our stuff in this tiny space. and it fit. it's our home. and again, why did we ever need anything more. of course, anyone takes a dump, and we all feel it. and we're never far enough away when grandma starts cooking broccoli, but its all part of the experience. brooke, the other day, asked grandma if she could bring her up some dinner, to which she replied, "only if it's healthy and nutritious." sometimes i get flashes of the movie "duplex" in my head with her (you'll have to see the movie), and then she does things like sew up my 40 year old coat that i stole from my dad, and that softens me up again. but she keeps us laughing.
so most of the time i'll just write a quick review of what's been going on in my day, but for now i'm trying to play a little catch up. so quick catch up: last semester was my first semester, and i took biology, chem prep, college algebra and trig. i ended up with a 4.0, including highest in my class in algebra and trig. who knew i'd be good at math. i was really nervous. but school is the easy part. the hard part is all of the extracurricular stuff that you are expected to do as a premed. you need community service, patient care experience, research, leadership, and physician shadowing. so last semester i began working at the ER. its pretty trivial work. i pass out blankets to the patients and take patients to and from scans. but it exposes me to life in the hospital, and it's really interesting. i try to keep busy, so i've invented a lot of things to do there, and have got to peek in on a couple of traumas. it seems like an eternity until i'll actually be doing this kind of stuff. but if i ever get down on myself, a warm blanket is always close by to comfort me. at the end of the semester, i discovered the cancer wellness house, where i'm now doing community service. mostly handyman stuff and organizing. they need it. but i get to kind of be alone with my thoughts and make things better than i found them, so i love it. plus its about a five block walk down through some old homes, so i enjoy the time away from campus. campus is beautiful, by the way, with lots of trees and old buildings.
so this semester, i'm in biology evolution, gen chem I, and calculus. so far things are going well, i got 100 percent on all of my first exams, so i'm well on my way to my next 4.0. if i don't pass out from exhaustion first. yesterday, i went to an interview for a research opportunity at the huntsman cancer institute. my friend dustin referred me to one of his buddies from grad school. he was hesitant at first, about hiring an undergrad, with little to know lab experience. but we started talking and he found out that i had paid attention in my classes, and started to warm up. he told me he was starting some research on MS, which is obviously close to my heart, and when i told him my dad has MS, he got really interested. he wants someone he can train and then set loose to do his own work. thats exactly what i want. i don't want to be someone's lab rat, i want to do my own project, discover things, get published. i felt really good about this meeting, and can't wait to get started. i was talking to myself, laughing, punching the air...all the way to the bus stop. great meeting. of course this means more time out of my schedule. i'm probably going to have to start studying on saturdays now. but this is what i have to do. but i'll be doing that probably 8 to 10 hours a week, with ER 4 hours a week, and cancer wellness house 2 hours a week. i'll probably be a TA next semester which is another 8 hours a week. in addition to that, i'm becoming involved in AED, the premed honors society, and am the new VP of public relations. designing flyers, posters, recruiting, events... lots of extra work, and the student advisor wants me to run for Vice President of the charter at the end of the semester and then be president the next year. its good for the resume, so i'm sure i'll do it. i don't know how i'll fit it all in and still have time to study and be with my family, but as i'm discovering, a way always presents itself.
so anyway, as this blog title suggests, i'm tired. i almost passed out on the bus earlier this week. i don't know what that was, but i'm sure sleep played a factor. brookie and i stayed up too late last night (11:45), so 5:20 came way too soon. but the weekend is upon me. only a couple classes and a few dozen warm blankets stands in the way of me and my family for a two day mini-vacation. can't wait.